Establishment of an Experimental System for Intraperitoneal Chemotherapy in a Rat Model

xlomafota13 shared this article with you from Inoreader Establishment of an Experimental System for Intraperitoneal Chemotherapy in a Rat Model Via In vivo (Athens, Greece) In Vivo. 2021 Sep-Oct;35(5):2703-2710. doi: 10.21873/invivo.12554. ABSTRACT AIM: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. MATERIALS AND METHODS: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. RESULTS: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. CONCLUSION: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans. PMID:34410959 | DOI:10.21873/invivo.12554 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.