Humoral immune response to authentic circulating SARS‐CoV‐2 variants elicited by booster vaccination with distinct RBD subunits in mice

alexandrossfakianakis shared this article with you from Inoreader Humoral immune response to authentic circulating SARS‐CoV‐2 variants elicited by booster vaccination with distinct RBD subunits in mice via Journal of Medical Virology Abstract SARS-CoV-2 variants could induce immune escape by mutations of the spike protein which are threatening to weaken vaccine efficacy. A booster vaccination is expected to increase the humoral immune response against SARS-CoV-2 variants in the population. We showed that immunization with two doses of wild type receptor-binding domain (RBD) protein, and booster vaccination with wild type or variant RBD protein all significantly increased binding and neutralizing antibody titers against wild type SARS-CoV-2 and its variants in mice. Only the booster immunization by Omicron (BA.1)RBD induced a strong antibody titer against the omicron virus strain and comparable antibody titers against all the other virus strains. These findings might shed the light on COVID-19 booster immunogens. This article is protected by copyright. All rights reserved. View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

COVID Salivary diagnostics: a comparative technical study

alexandrossfakianakis shared this article with you from Inoreader COVID Salivary diagnostics: a comparative technical study via Journal of Medical Virology ABSTRACT Since the beginning of the COVID-19 pandemic, molecular diagnostics of SARS-CoV-2 have taken center stage in the detection of infected individuals for isolation purposes but also in the mass surveillance as a preventive strategy to contain the virus spread. While nasopharyngeal swabs (NPS) have remained the golden standard substrate, salivary diagnostic for SARS-CoV-2 has been proposed as an alternative and non-invasive measure in vulnerable individuals. Nevertheless, there is a widespread assumption that salivary RT-PCR does not match the quality of testing using NPS and particular care should be taken in respect to food or beverage intake, when sampling saliva. Our study indicates that without any precaution in the selection of 190 patients, nor restriction over the time-window of sampling, there is 99% match in the COVID-19 positivity between NPS and saliva when using RT-PCR, with a reported Delta in thermal cycles (Cts) values for the viral genes Envelop e (E) and Open reading frame 1ab (Orf1ab) between 0 and 2, a 98.7% Sensitivity and 100% Specificity. This high accuracy is maintained in pooling configurations that can be used for mass-testing purposes in professional and educational settings. The further advantage to using crude saliva as compared to NPS or mouthwash is that direct methods yield robust results. Overall, our study validates and promotes the use of salivary diagnostic for COVID-19 eliminating the need of a medical practitioner for the sampling, resolving the unpleasantness of the NPS intervention and empowering the patient to do self-testing in times of need. This article is protected by copyright. All rights reserved. View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Carbohydrate quantity is more closely associated with glycaemic control than weight in pregnant women with type 1 diabetes; insights from the Diabetes and Pre‐eclampsia Intervention Trial (DAPIT)

alexandrossfakianakis shared this article with you from Inoreader Carbohydrate quantity is more closely associated with glycaemic control than weight in pregnant women with type 1 diabetes; insights from the Diabetes and Pre‐eclampsia Intervention Trial (DAPIT) via Journal of Human Nutrition and Dietetics Abstract Aim To explore the relationships between carbohydrate intake, body mass index and glycaemic control (HbA1c) in pregnant women with type 1 diabetes mellitus (T1DM) Methods Secondary analysis of data was undertaken to assess dietary intake in a cohort of women who participated in a randomized controlled trial (RCT) of antioxidant supplementation to prevent pre-eclampsia (DAPIT10). Study specific peripheral venous blood samples were obtained for HbA1c at 26 weeks and 34 weeks. Diet was collected using a validated semi-quantitative food frequency questionnaire at 26-28 weeks gestation which assessed dietary intake over 2 weeks. Mean daily average nutrient intakes were analysed using Q Builder nutritional software (Tinuviel Software, UK) and SPSS Version 25). Results Dietary data were available for 547 pregnant women (72% of cohort) aged 29 years (95% CI 28.9-29.9) with average diabetes duration 11.8 years (95% CI 11.1-12.6). Averag e BMI (<16 weeks gestation) was 26.7 kg/m2 (95% CI 26.3 -27 range 18.8-45.6 kg/m2); 43% (n=234) were overweight (BMI 25.0 - 29.9kg/m2) and 20% (n= 112) were obese (BMI>30kg/m2). Differences in HbA1c and carbohydrate quantity and quality were found when adjusted for age and insulin dose. No differences between BMI group were observed in total carbohydrate and glycaemic control, however, differences were noted in fibre and glycaemic index. Conclusion Average quantity of dietary carbohydrate influenced HbA1c when adjusted for insulin dose however, BMI had less impact. More research is required on the relationship between carbohydrate consumption and glycaemic control in pregnancy. This article is protected by copyright. All rights reserved. View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Septic shock with Chlamydia abortus infection

alexandrossfakianakis shared this article with you from Inoreader Septic shock with <em>Chlamydia abortus</em> infection via ScienceDirect Publication: The Lancet Infectious Diseases Publication date: June 2022 Source: The Lancet Infectious Diseases, Volume 22, Issue 6 Author(s): Mengting Liu, Yin Wen, Hongguang Ding, Hongke Zeng View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Activin A downregulates the CD69-MT2A axis via p38MAPK to induce erythroid differentiation that sensitizes BCR-ABL-positive cells to imatinib

alexandrossfakianakis shared this article with you from Inoreader Activin A downregulates the CD69-MT2A axis via p38MAPK to induce erythroid differentiation that sensitizes BCR-ABL-positive cells to imatinib via ScienceDirect Publication: Experimental Cell Research Publication date: Available online 26 May 2022 Source: Experimental Cell Research Author(s): Chih-Wei Chen, Nai-Kuei Huang, Yueh-Lun Lee, Chia-Kwung Fan, Yen-Chou Chen, Chin-Wei Liu, Huei-Mei Huang View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Pediatric Button Battery Injuries—Current State and What’s Next?

alexandrossfakianakis shared this article with you from Inoreader Pediatric Button Battery Injuries—Current State and What's Next? via JAMA Otolaryngology–Head & Neck Surgery Online First The study by Philteos et al in this issue of JAMA Otolaryngology–Head & Neck Surgery is a systematic review of published cases of airway injury among pediatric patients who ingested button batteries (BBs). Although these types of severe complications associated with BB ingestion have been previously reported, to date this is the largest review focused on airway injuries. Given the direct proximity of the esophagus and the trachea, airway injury is a possible risk in cases of BB ingestion. The authors' review of the literature and dat a from the National Capital Poison Center (NCPC) database yielded 195 cases total. The mean age at BB ingestion was 17.8 months, and the mean time between ingestion and removal was 5.8 days. The duration of impaction is difficult to precisely determine because many ingestions are unwitnessed and have a delayed diagnosis. Button batteries at least 20 mm in diameter (with lithium chemistry) are more likely to become impacted in the pediatric esophagus, but smaller BBs (including those with nonlithium chemistry) also pose a substantial risk; 11% of the injury cases with known BB diameters in this report involved BBs smaller than 20 mm, with the smallest diameter being 10 mm. Tracheoesophageal fistula (TEF), a life-threatening complication of BB ingestion, was found to be the most common airway injury (79%). Most TEFs required surgical management, and all 14 deaths reported TEF involvement. Other airway complications found included unilateral and bilateral vocal cord paralysis, subglott ic stenosis, tracheal stenosis, and tracheomalacia. The use of injury mitigation strategies before or after BB removal was not assessed in this study, which is limited by the known underreporting of these cases to any data source. This review by Philteos et al describes complications from BB ingestions associated with airway injury, invaluably highlighting a significant source of pediatric morbidity and mortality. View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Changes in Cone-Beam CT Pediatric Airway Measurements After Adenotonsillectomy in Patients With OSA

alexandrossfakianakis shared this article with you from Inoreader Changes in Cone-Beam CT Pediatric Airway Measurements After Adenotonsillectomy in Patients With OSA via JAMA Otolaryngology–Head & Neck Surgery Online First This cohort study assesses changes in cone-beam computed tomog raphy airway measurements that occur after adenotonsillectomy in children with obstructive sleep apnea and examines whether these changes are correlated with apnea-hypopnea index reduction. View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Concomitant and Exceedingly Rare Causes of Oropharyngeal Dysphagia

alexandrossfakianakis shared this article with you from Inoreader Concomitant and Exceedingly Rare Causes of Oropharyngeal Dysphagia via JAMA Otolaryngology–Head & Neck Surgery Online First This case report describes a man in his 40s who presented with a 5-month history of worsening dysphonia and dysphagia and was subsequently diagnosed with extranodal natural killer/T-cell lymphoma. View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Airway Complications Resulting From Pediatric Esophageal Button Battery Impaction

alexandrossfakianakis shared this article with you from Inoreader Airway Complications Resulting From Pediatric Esophageal Button Battery Impaction via JAMA Otolaryngology–Head & Neck Surgery Online First This systematic review investigates pediatric airway injuries and their long-term sequalae after button battery ingestion. View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Magnetic resonance guided adaptive stereotactic body radiotherapy for lung tumors in ultracentral location: the MAGELLAN trial (ARO 2021-3)

alexandrossfakianakis shared this article with you from Inoreader Magnetic resonance guided adaptive stereotactic body radiotherapy for lung tumors in ultracentral location: the MAGELLAN trial (ARO 2021-3) via Radiation Oncology - Latest Articles Stereotactic Body Radiotherapy (SBRT) is a standard treatment for inoperable primary and secondary lung tumors. In case of ultracentral tumor location, defined as tumor contact with vulnerable mediastinal stru... View on Web

Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.