Thursday, July 21, 2022

Oral microbiome diversity – The curious case of Corynebacterium sp. isolation

alexandrossfakianakis shared this article with you from Inoreader

Abstract

Oral microbiome sequencing efforts revealed the presence of hundreds of different microbes. Interindividual differences at strain and species resolution suggests that microbiome diversity could lead to mechanistically distinct gene regulation as well as species related differences in phenotypes. Commonly, gene regulation and related phenotypes are studied in a few selected strains of a particular species with conclusions that are mostly generalized. The aim of this study was to isolate several species of Corynebacterium using an established protocol that led to the previous isolation of C. durum. Characterization of C. durum interspecies interactions revealed a specific mechanism for chain elongation in Streptococcus sanguinis that was the result of corynebacterial fatty acid production and secretion. While the protocol was successful applied to isolate what we presumed to be additional Corynebacterium based on several phenotypic traits that seem to be identical to C. durum, genome sequencing of the newly isolated strains placed them closer to Actinomyces. Both Corynebacterium and Actinomyces are sub-orders of the Actino-bacteridae and related species. Our study suggests to take several comprehensive strategies into consideration when taxonomically identifying closely related microorganisms. Furthermore, it seems to be important to test common core phenotypes in bacterial ecology to understand the behavior of specific groups of microbes, rather than simply relying upon genome sequence homology to establish relationships in the microbiome.

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Associations of Prior Head Injury With Olfaction in Older Adults

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This cohort study investigates the associations of prior head injury, number of prior head injuries, and head injury severity with subjective and psychophysical olfactory function in older adults.
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Evaluate the safety and efficacy of dura sealant patch in reducing cerebrospinal fluid leakage following elective cranial surgery (ENCASE II): study protocol for a randomized, two-arm, multicenter trial

alexandrossfakianakis shared this article with you from Inoreader

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Cerebrospinal fluid (CSF) leakage is a frequent and challenging complication in neurosurgery, especially in the posterior fossa, with a prevalence of 8%. It is associated with substantial morbidity and increas...
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ELF4 is a critical component of a miRNA-transcription factor network and is a bridge regulator of glioblastoma receptor signaling and lipid dynamics

alexandrossfakianakis shared this article with you from Inoreader
Abstract
Background
The loss of neurogenic tumor suppressor microRNAs miR-124, miR-128, and miR-137 is associated with glioblastoma's undifferentiated state. Most of their impact comes via the repression of a network of oncogenic transcription factors. We conducted a high-throughput functional siRNA screen in glioblastoma cells and identify E74 like ETS transcription factor 4 (ELF4) as the leading contributor to oncogenic phenotypes.
Methods
In vitro and in vivo assays were used to assess ELF4 impact on cancer phenotypes. We characterized ELF4's mechanism of action via genomic and lipidomic analyses. A MAPK reporter assay verified ELF4's impact on MAPK signaling, and qRT-PCR and western blotting were used to corroborate ELF4 regulatory role on most relevant target genes.
Results
ELF4 knockdown resulted in significant proliferation delay and ap optosis in GBM cells and long-term growth delay and morphological changes in glioma stem cells (GSCs). Transcriptomic analyses revealed that ELF4 controls two interlinked pathways: 1) Receptor tyrosine kinase signaling, and 2) Lipid dynamics. ELF4 modulation directly affected Receptor Tyrosine Kinase (RTK) signaling, as mitogen-activated protein kinase (MAPK) activity was dependent upon ELF4 levels. Furthermore, shotgun lipidomics revealed that ELF4 depletion disrupted several phospholipid classes, highlighting ELF4's importance in lipid homeostasis.
Conclusions
We found that ELF4 is critical for the GBM cell identity by controlling genes of two dependent pathways: RTK signaling (SRC, PTK2B, TNK2) and lipid dynamics (LRP1, APOE, ABCA7, PLA2G6, and PITPNM2). Our data suggests that targeting these two pathways simultaneously may be therapeutically beneficial to GBM patients.
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Evaluation of facial soft tissue asymmetric changes in Class III patients after orthognathic surgery using three-dimensional stereophotogrammetry

alexandrossfakianakis shared this article with you from Inoreader
The aim of this study was to investigate changes in facial soft tissue asymmetry over time after orthognathic surgery in Class III patients using three-dimensional stereophotogrammetry. The study included 101 patients with a skeletal Class III malocclusion (72 female, 29 male; age range 19 –53 years, mean age 28.6 years) who underwent orthognathic surgery. The minimum follow-up was 12 months. Three-dimensional photographs were acquired using the 3dMDtrio stereophotogrammetry system, and 21 anthropometric landmark positions were evaluated at three time points: before surgery (T0), 6 months (T1) and 12 months (T2) after surgery. (Source: International Journal of Oral and Maxillofacial Surgery)
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Three-dimensional evaluation of postoperative stability: a comparative study between surgery-first and surgery-late protocols

alexandrossfakianakis shared this article with you from Inoreader
The main objective of this study was to compare the stability of the surgery-first and surgery-late approaches according to the standardized centre protocols, by three-dimensional evaluation after 1 year of follow-up. A retrospective study was designed that included a test group (surgery-first protocol) and a control group (surgery-late protocol), with a follow-up period of at least 1 year (average 14 months; range 12 –24 months). Stability was evaluated using linear and angular measurements by superimposing cone beam computed tomography images obtained at specific points in time: preoperatively, 1 month after surgery, and at the end of the orthodontic treatment. (Source: International Journal of Oral and Maxillofacial Surgery)
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