New insight into the role of ANXA1 in melanoma progression: involvement of stromal expression in dissemination

xlomafota13 shared this article with you from Inoreader New insight into the role of ANXA1 in melanoma progression: involvement of stromal expression in dissemination Via American Journal of Cancer Research Am J Cancer Res. 2021 Apr 15;11(4):1600-1615. eCollection 2021. ABSTRACT ANXA1, first described in the context of inflammation, appears to be deregulated in many cancers and increased in melanomas compared with melanocytes. To date, few studies have investigated the role of ANXA1 in melanoma progression. Furthermore, this protein is expressed by various cell types, including immune and endothelial cells. We therefore analyzed the specific roles of ANXA1 using melanoma and stromal cells in two human cell lines (A375-MA2 and SK-MEL-28) in vitro and in Anxa1 null C57Bl6/J mice bearing B16Bl6 tumors. We report decreased proliferation in both ANXA1 siRNA A375-MA2 and SK-MEL-28, but cell-dependent effects of ANXA1 in migration in vitro. However, we also observed a significant decrease of B16Bl6 tumor growth associated with a reduction of Ki-67 positive cells in Anxa1 null mice compared with wild-type mice. Interes tingly, we also found a significant reduction of spontaneous metastases, which can be attributed to decreased angiogenesis concomitantly with greater immune cell presence in the Anxa1 null stromal context. This study highlights the pejorative role of ANXA1 in both tumor and stromal cells in melanoma, due to its involvement in proliferation and angiogenesis. PMID:33948376 | PMC:PMC8085877 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.