Thursday, February 28, 2019

Chimeric crRNAs with 19 DNA residues in the guide region show retained DNA cleavage activity of Cas9 with a potential to improve the specificity

Chem. Commun., 2019, Accepted Manuscript
DOI: 10.1039/C8CC08468H, Communication
Dae-Ro Ahn, Hyo Young Kim, Jinsu An, Hee Jae Lee, Jeong-Eun Jang, Yongmoon Jeon, Seong Jae Kang, Hak Suk Chung, Cherlhyun Jeong, Duhee Bang, JongSeong Ahn, Jihyun Park
We demonstrated that 19 out of 20 RNA residues in the guide region of crRNA can be replaced by DNA residues with high GC-contents. The cellular activity of the chimeric...
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