Direct Tissue PCR and Genotyping for Species Identification in a Case of Laryngeal Blastomycosis

xlomafota13 shared this article with you from Inoreader Direct Tissue PCR and Genotyping for Species Identification in a Case of Laryngeal Blastomycosis Via ear nose throat Ear Nose Throat J. 2021 Mar 8:145561321991342. doi: 10.1177/0145561321991342. Online ahead of print. ABSTRACT Otolaryngologic manifestations of infection with Blastomyces species are extremely rare and restricted geographically to recognized endemic regions. Here, we describe a case of laryngeal blastomycosis that presented as slowly progressive dysphonia. While a preliminary diagnosis was made using routine histopathology, a species identification of Blastomyces d ermatitidis was made using polymerase chain reaction amplification and rapid genotyping without the need for fungal culture. All symptoms resolved following 1 month of antifungal therapy. Rapid molecular differentiation of B dermatitidis from Blastomyces gilchristii provides important insights into pathogenesis given recent recognition of differences in clinical spectra. PMID:33683980 | DOI:10.1177/0145561321991342 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.