Effect of the orientation of microskin on the survival rate of transplantation and improving the method.

xlomafota13 shared this article with you from Inoreader Effect of the orientation of microskin on the survival rate of transplantation and improving the method. Via International Journal of Clinical and Experimental Pathology Related Articles Effect of the orientation of microskin on the survival rate of transplantation and improving the method. Int J Clin Exp Pathol. 2021;14(2):186-195 Authors: Zheng X, Li X, Chen T, Chang F, Ji S, Hu X, Xiao S Abstract The direction of microskin transplantation is difficult to control, and the survival rate is critically affected. In this study, we show for the first time that survival rate of transplantation was improved by changing the direction of microskin. A human split-thickness skin graft was prepared as microskin (size of 1 mm × 1 mm), and was transplanted onto a wound in nude mice. The effect of the orientation of microskin on the survival rate of transplants was observed. The collagen membrane was first attached to the epidermal surface of pig skin, which was then cut into microskin and then they were floated on physiological saline. The effect of the collagen membrane on the orientation of microskin was observed. Then the microskin of pig with an epidermal surface attached to the collagen membrane was transplanted to the wound of the pig, and the survival rate of transplants was observed. In the 2nd, 3rd and 4th week after transplantation of nude mice, the wound healing rate in group A (all of the microskin's epidermal surface was upward) was significantly higher than in other groups (P < 0.01). The floating rate and the forward floating rate in the experimental group were significantly higher than those in the control group (P < 0.01). Four weeks after microskin transplantation of pigs, the wound contraction rate in group A, compared with group B, was significantly lower, and the wound healing rate was significantly higher (P < 0.01). In microskin grafting, the direction of microskin significantly affects the survival rate of transplantation. The method of adhering the collagen membrane to the epidermal surface of microskin may ensure complete floating of microskin on the physiological saline with the epidermal surface facing up. This is a new method to improve the survival rate of microskin grafting. PMID: 33564351 [PubMed] View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.